GAA ELISA Kit

Produktnummer ABIN5654631

Produktdetails GAA ELISA Kit

Target: GAA (Glucosidase, Alpha, Acid (GAA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.625 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.625 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Glucosidase Alpha, Acid (GaA). No significant cross-reactivity or interference between Glucosidase Alpha, Acid (GaA) and analogues was observed.

Sensitivität: 0.238 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucosidase Alpha, Acid (GaA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucosidase Alpha, Acid (GaA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucosidase Alpha, Acid (GaA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucosidase Alpha, Acid (GaA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucosidase Alpha, Acid (GaA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucosidase Alpha, Acid (GaA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GAA

Target: GAA (Glucosidase, Alpha, Acid (GAA))

Andere Bezeichnung: Glucosidase Alpha, Acid (GAA Produkte)

Synonyme: LYAG ELISA Kit, E430018M07Rik ELISA Kit, glucosidase alpha, acid ELISA Kit, glucosidase, alpha, acid ELISA Kit, transmembrane and coiled-coil domain family 1 ELISA Kit, glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II) ELISA Kit, GAA ELISA Kit, Gaa ELISA Kit, TMCC1 ELISA Kit, gaa ELISA Kit

Hintergrund:

Gene Name: Glucosidase Alpha, Acid

Gene Aliases: LYAG, Acid Alpha-Glucosidase, Lysosomal Alpha-Glucosidase, Pompe Disease Glycogen Storage Disease Type II, Acid Maltase, Aglucosidase Alfa

Gen-ID: 2548

UniProt: P10253

Pathways: Cellular Glucan Metabolic Process