GRIN1/NMDAR1 ELISA Kit

Produktnummer ABIN5654667

Produktdetails GRIN1/NMDAR1 ELISA Kit

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1). No significant cross-reactivity or interference between Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) and analogues was observed.

Sensitivität: 0.64 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GRIN1/NMDAR1

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Andere Bezeichnung: Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1 (GRIN1 Produkte)

Synonyme: GluN1 ELISA Kit, MRD8 ELISA Kit, NMDA1 ELISA Kit, NMDAR1 ELISA Kit, NR1 ELISA Kit, nmda1 ELISA Kit, nmdar1 ELISA Kit, GB19253 ELISA Kit, GRIN1 ELISA Kit, NMD-R1 ELISA Kit, Z16 ELISA Kit, GluRdelta1 ELISA Kit, GluRzeta1 ELISA Kit, M100174 ELISA Kit, Nmdar ELISA Kit, Rgsc174 ELISA Kit, NMDAR1.1 ELISA Kit, zgc:152678 ELISA Kit, glutamate ionotropic receptor NMDA type subunit 1 ELISA Kit, glutamate receptor, ionotropic, N-methyl D-aspartate 1 S homeolog ELISA Kit, NMDA receptor 1 ELISA Kit, G protein-regulated inducer of neurite outgrowth 1 ELISA Kit, glutamate receptor, ionotropic, NMDA1 (zeta 1) ELISA Kit, glutamate receptor, ionotropic, N-methyl D-aspartate 1a ELISA Kit, GRIN1 ELISA Kit, grin1.S ELISA Kit, grin1 ELISA Kit, Grin1 ELISA Kit, Nmdar1 ELISA Kit, Gprin1 ELISA Kit, grin1a ELISA Kit

Hintergrund:

Gene Name: Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1

Gene Aliases: NMDA1, NMDAR1, NR1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity