GSTM3 ELISA Kit

Produktnummer ABIN5654715

Produktdetails GSTM3 ELISA Kit

Target: GSTM3 (Glutathione S-Transferase mu 3 (Brain) (GSTM3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12 ng/mL - 200 ng/mL

Untere Nachweisgrenze: 3.12 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Glutathione S Transferase Mu 3, Brain (GSTm3). No significant cross-reactivity or interference between Glutathione S Transferase Mu 3, Brain (GSTm3) and analogues was observed.

Sensitivität: 1.15 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Mu 3, Brain (GSTm3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Mu 3, Brain (GSTm3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Mu 3, Brain (GSTm3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione S Transferase Mu 3, Brain (GSTm3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione S Transferase Mu 3, Brain (GSTm3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione S Transferase Mu 3, Brain (GSTm3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GSTM3

Target: GSTM3 (Glutathione S-Transferase mu 3 (Brain) (GSTM3))

Andere Bezeichnung: Glutathione S Transferase Mu 3, Brain (GSTM3 Produkte)

Synonyme: GST5 ELISA Kit, GSTB ELISA Kit, GSTM3-3 ELISA Kit, GTM3 ELISA Kit, Fsc2 ELISA Kit, mGSTM5 ELISA Kit, glutathione S-transferase mu 3 ELISA Kit, glutathione S-transferase Mu 3 ELISA Kit, glutathione S-transferase, mu 3 ELISA Kit, GSTM3 ELISA Kit, Gstm3 ELISA Kit, LOC479911 ELISA Kit

Hintergrund:

Gene Name: Glutathione S Transferase Mu 3, Brain

Gene Aliases: GST-M3, GST5, GSTB, GSTM3-3, GTM3

Gen-ID: 2947

UniProt: P21266