GLDC CLIA Kit

Produktnummer ABIN5654738

Produktdetails GLDC CLIA Kit

Target: GLDC (Glycine Dehydrogenase (GLDC))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 156.25 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156.25 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Glycine Dehydrogenase (GLDC). No significant cross-reactivity or interference between Glycine Dehydrogenase (GLDC) and analogues was observed.

Sensitivität: 4.7 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Glycine Dehydrogenase (GLDC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Glycine Dehydrogenase (GLDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Glycine Dehydrogenase (GLDC) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycine Dehydrogenase (GLDC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycine Dehydrogenase (GLDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: GLDC (Glycine Dehydrogenase (GLDC))

Andere Bezeichnung: Glycine Dehydrogenase (GLDC Produkte)

Synonyme: PSPTO1276 CLIA Kit, Tb07.43M14.350 CLIA Kit, DDBDRAFT_0219205 CLIA Kit, DDBDRAFT_0231130 CLIA Kit, DDB_0219205 CLIA Kit, DDB_0231130 CLIA Kit, D030049L12Rik CLIA Kit, D19Wsu57e CLIA Kit, GCE CLIA Kit, GCSP CLIA Kit, HYGN1 CLIA Kit, fb23b05 CLIA Kit, wu:fb23b05 CLIA Kit, zgc:66198 CLIA Kit, gldc CLIA Kit, glycine dehydrogenase (decarboxylating) GcvP CLIA Kit, glycine dehydrogenase CLIA Kit, glycine cleavage system P-protein CLIA Kit, glycine decarboxylase CLIA Kit, glycine dehydrogenase (decarboxylating) CLIA Kit, glycine decarboxylase S homeolog CLIA Kit, gcvP CLIA Kit, Tb927.7.1910 CLIA Kit, Gldc CLIA Kit, GLDC CLIA Kit, gldc CLIA Kit, gldc.S CLIA Kit

Hintergrund:

Gene Name: Glycine Dehydrogenase

Gene Aliases: GCSP, NKH, Decarboxylating, Glycine Decarboxylase, Glycine Cleavage System Protein P, Glycine dehydrogenase (aminomethyl-transferring)

Gen-ID: 2731

UniProt: P23378