GARS ELISA Kit

Produktnummer ABIN5654774

Produktdetails GARS ELISA Kit

Target: GARS (Glycyl-tRNA Synthetase (GARS))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Glycyl tRNA Synthetase (GARS). No significant cross-reactivity or interference between Glycyl tRNA Synthetase (GARS) and analogues was observed.

Sensitivität: 6.6 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycyl tRNA Synthetase (GARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycyl tRNA Synthetase (GARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycyl tRNA Synthetase (GARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycyl tRNA Synthetase (GARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycyl tRNA Synthetase (GARS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycyl tRNA Synthetase (GARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GARS

Target: GARS (Glycyl-tRNA Synthetase (GARS))

Andere Bezeichnung: Glycyl tRNA Synthetase (GARS Produkte)

Synonyme: CMT2D ELISA Kit, DSMAV ELISA Kit, GlyRS ELISA Kit, HMN5 ELISA Kit, SMAD1 ELISA Kit, Aat-gly ELISA Kit, CG6778 ELISA Kit, Dmel\\CG6778 ELISA Kit, GRS ELISA Kit, gars ELISA Kit, team ELISA Kit, RGD1559871 ELISA Kit, GENA202 ELISA Kit, Gena201 ELISA Kit, Nmf249 ELISA Kit, Sgrp23 ELISA Kit, GB13467 ELISA Kit, MGC79495 ELISA Kit, fb02f03 ELISA Kit, si:dkey-276i5.1 ELISA Kit, wu:fb02f03 ELISA Kit, NI36_08435 ELISA Kit, glycyl-tRNA synthetase ELISA Kit, Glycyl-tRNA synthetase ELISA Kit, glycine--tRNA ligase ELISA Kit, glycyl-tRNA synthetase L homeolog ELISA Kit, Glycine--tRNA ligase ELISA Kit, GARS ELISA Kit, GlyRS ELISA Kit, Gars ELISA Kit, LOC408392 ELISA Kit, gars.L ELISA Kit, gars ELISA Kit, LOC692991 ELISA Kit, glyQ ELISA Kit, TTHA0543 ELISA Kit, NI36_RS08405 ELISA Kit

Hintergrund:

Gene Name: Glycyl tRNA Synthetase

Gene Aliases: CMT2D, CMT2-D, DSMAV, GlyRS, SMAD1, EJ, Charcot-Marie-Tooth Neuropathy 2D, Glycine tRNA Ligase, Diadenosine tetraphosphate synthetase, AP-4-A synthetase

Gen-ID: 2617

UniProt: P41250

Pathways: Ribonucleoside Biosynthetic Process