GRAP2 ELISA Kit

Produktnummer ABIN5654821

Produktdetails GRAP2 ELISA Kit

Target: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of GRB2 Related Adaptor Protein 2 (GRAP2). No significant cross-reactivity or interference between GRB2 Related Adaptor Protein 2 (GRAP2) and analogues was observed.

Sensitivität: 0.061 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GRB2 Related Adaptor Protein 2 (GRAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GRB2 Related Adaptor Protein 2 (GRAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GRB2 Related Adaptor Protein 2 (GRAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GRB2 Related Adaptor Protein 2 (GRAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GRB2 Related Adaptor Protein 2 (GRAP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GRB2 Related Adaptor Protein 2 (GRAP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu GRAP2

Target: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Andere Bezeichnung: GRB2 Related Adaptor Protein 2 (GRAP2 Produkte)

Synonyme: GADS ELISA Kit, GRAP-2 ELISA Kit, GRB2L ELISA Kit, GRBLG ELISA Kit, GRID ELISA Kit, GRPL ELISA Kit, GrbX ELISA Kit, Grf40 ELISA Kit, Mona ELISA Kit, P38 ELISA Kit, Gads ELISA Kit, GrpL ELISA Kit, zgc:112091 ELISA Kit, GRAP2 ELISA Kit, grap2 ELISA Kit, zgc:65942 ELISA Kit, GRB2-related adaptor protein 2 ELISA Kit, GRB2-related adaptor protein 2a ELISA Kit, GRB2-related adaptor protein 2b ELISA Kit, GRAP2 ELISA Kit, Grap2 ELISA Kit, grap2a ELISA Kit, grap2 ELISA Kit, grap2b ELISA Kit

Hintergrund:

Gene Name: GRB2 Related Adaptor Protein 2

Gene Aliases: P38, GADS, GRAP-2, GRB2L, GRBLG, GRID, GRPL, GrbX, Grf40, Mona, Growth factor receptor-binding protein, Hematopoietic cell-associated adapter protein GrpL

Gen-ID: 9402

UniProt: O75791

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung