HMOX2 ELISA Kit
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- Target Alle HMOX2 ELISA Kits anzeigen
- HMOX2 (Heme Oxygenase (Decycling) 2 (HMOX2))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.156 ng/mL - 10 ng/mL
- Untere Nachweisgrenze
- 0.156 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Heme Oxygenase 2, Decycling (HO2). No significant cross-reactivity or interference between Heme Oxygenase 2, Decycling (HO2) and analogues was observed.
- Sensitivität
- 0.059 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heme Oxygenase 2, Decycling (HO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heme Oxygenase 2, Decycling (HO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heme Oxygenase 2, Decycling (HO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heme Oxygenase 2, Decycling (HO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heme Oxygenase 2, Decycling (HO2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heme Oxygenase 2, Decycling (HO2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle HMOX2 ELISA Kits anzeigen
- HMOX2 (Heme Oxygenase (Decycling) 2 (HMOX2))
- Andere Bezeichnung
- Heme Oxygenase 2, Decycling (HMOX2 Produkte)
- Synonyme
- HO-2 ELISA Kit, HO2 ELISA Kit, HMOX2 ELISA Kit, Ho-2 ELISA Kit, im:6909100 ELISA Kit, si:dkey-44g23.7 ELISA Kit, AtHO2 ELISA Kit, T9J22.22 ELISA Kit, T9J22_22 ELISA Kit, heme oxygenase 2 ELISA Kit, hmox2 ELISA Kit, ho-2 ELISA Kit, heme oxygenase 2 ELISA Kit, Heme oxygenase 2 ELISA Kit, heme oxygenase 2, putative ELISA Kit, heme oxygenase 2a ELISA Kit, heme oxygenase 2 L homeolog ELISA Kit, HMOX2 ELISA Kit, Hmox2 ELISA Kit, HO2 ELISA Kit, ho2 ELISA Kit, CNF01200 ELISA Kit, CPR_0203 ELISA Kit, CGB_F5120W ELISA Kit, hmox2a ELISA Kit, hmox2.L ELISA Kit
- Hintergrund
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Gene Name: Heme Oxygenase 2, Decycling
Gene Aliases: HMOX2
- Gen-ID
- 3163
- UniProt
- P30519
- Pathways
- Transition Metal Ion Homeostasis
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