HBA1 ELISA Kit

Produktnummer ABIN5655027

Produktdetails HBA1 ELISA Kit

Target: HBA1 (Hemoglobin, alpha 1 (HBA1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Hemoglobin Alpha 1 (HBa1). No significant cross-reactivity or interference between Hemoglobin Alpha 1 (HBa1) and analogues was observed.

Sensitivität: 0.63 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin Alpha 1 (HBa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemoglobin Alpha 1 (HBa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemoglobin Alpha 1 (HBa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin Alpha 1 (HBa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemoglobin Alpha 1 (HBa1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemoglobin Alpha 1 (HBa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu HBA1

Target: HBA1 (Hemoglobin, alpha 1 (HBA1))

Andere Bezeichnung: Hemoglobin Alpha 1 (HBA1 Produkte)

Synonyme: HBA1 ELISA Kit, HBAM ELISA Kit, Hba-a1 ELISA Kit, Hba-a2 ELISA Kit, cd31 ELISA Kit, hba-a ELISA Kit, hba2 ELISA Kit, HBH ELISA Kit, HBA2 ELISA Kit, alpha globin ELISA Kit, CD31 ELISA Kit, Hba ELISA Kit, Hba1 ELISA Kit, Hbat1 ELISA Kit, AA1 ELISA Kit, dZ118J2.2 ELISA Kit, HBAI ELISA Kit, HBAII ELISA Kit, hemoglobin, alpha 1 ELISA Kit, hemoglobin subunit alpha 1 ELISA Kit, hemoglobin subunit alpha 1 S homeolog ELISA Kit, hemoglobin subunit alpha 2 ELISA Kit, hemoglobin, alpha 2 ELISA Kit, hemoglobin subunit alpha-like ELISA Kit, hemoglobin alpha, adult chain 1 ELISA Kit, alpha-hemoglobin ELISA Kit, hemoglobin subunit alpha ELISA Kit, hemoglobin, alpha adult 1 ELISA Kit, hemoglobin subunit alpha-2-like ELISA Kit, HBA ELISA Kit, Hba1 ELISA Kit, hba1 ELISA Kit, hba1.S ELISA Kit, HBA1 ELISA Kit, HBA2 ELISA Kit, LOC100737768 ELISA Kit, Hba-a1 ELISA Kit, LOC100472013 ELISA Kit, LOC100087130 ELISA Kit, hbaa1 ELISA Kit, LOC102168959 ELISA Kit

Hintergrund:

Gene Name: Hemoglobin Alpha 1

Gene Aliases: Alpha-globin

UniProt: P69905