HDLBP ELISA Kit

Produktnummer ABIN5655125

Produktdetails HDLBP ELISA Kit

Target: HDLBP (High Density Lipoprotein Binding Protein (HDLBP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of High Density Lipoprotein Binding Protein (HDLBP). No significant cross-reactivity or interference between High Density Lipoprotein Binding Protein (HDLBP) and analogues was observed.

Sensitivität: 0.056 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Density Lipoprotein Binding Protein (HDLBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Density Lipoprotein Binding Protein (HDLBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Density Lipoprotein Binding Protein (HDLBP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Density Lipoprotein Binding Protein (HDLBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu HDLBP

Target: HDLBP (High Density Lipoprotein Binding Protein (HDLBP))

Andere Bezeichnung: High Density Lipoprotein Binding Protein (HDLBP Produkte)

Synonyme: HBP ELISA Kit, PRO2900 ELISA Kit, VGL ELISA Kit, 1110005P14Rik ELISA Kit, AA960365 ELISA Kit, AI118566 ELISA Kit, D1Ertd101e ELISA Kit, HDLBP ELISA Kit, vigilin ELISA Kit, cb591 ELISA Kit, cb811 ELISA Kit, hdlbp ELISA Kit, wu:fb36d01 ELISA Kit, wu:fb94h08 ELISA Kit, wu:fc23d06 ELISA Kit, wu:fc75h07 ELISA Kit, high density lipoprotein binding protein ELISA Kit, high density lipoprotein (HDL) binding protein ELISA Kit, vigilin ELISA Kit, high density lipoprotein binding protein (vigilin) S homeolog ELISA Kit, high density lipoprotein binding protein a ELISA Kit, HDLBP ELISA Kit, Hdlbp ELISA Kit, hdlbp.S ELISA Kit, hdlbpa ELISA Kit

Hintergrund:

Gene Name: High Density Lipoprotein Binding Protein

Gene Aliases: HBP, VGL, Vigilin

Gen-ID: 3069

UniProt: Q00341