IGBP1 ELISA Kit

Produktnummer ABIN5655272

Produktdetails IGBP1 ELISA Kit

Target: IGBP1 (Immunoglobulin (CD79A) Binding Protein 1 (IGBP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Immunoglobulin Binding Protein 1 (IGBP1). No significant cross-reactivity or interference between Immunoglobulin Binding Protein 1 (IGBP1) and analogues was observed.

Sensitivität: 0.116 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin Binding Protein 1 (IGBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin Binding Protein 1 (IGBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin Binding Protein 1 (IGBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin Binding Protein 1 (IGBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin Binding Protein 1 (IGBP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin Binding Protein 1 (IGBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu IGBP1

Target: IGBP1 (Immunoglobulin (CD79A) Binding Protein 1 (IGBP1))

Andere Bezeichnung: Immunoglobulin Binding Protein 1 (IGBP1 Produkte)

Synonyme: ALPHA-4 ELISA Kit, IBP1 ELISA Kit, fc70b08 ELISA Kit, fi33h08 ELISA Kit, wu:fc70b08 ELISA Kit, wu:fi33h08 ELISA Kit, zgc:55347 ELISA Kit, AI115586 ELISA Kit, AI662457 ELISA Kit, AW208785 ELISA Kit, C81413 ELISA Kit, Pc52 ELISA Kit, Tap42 ELISA Kit, p52 ELISA Kit, MGC83250 ELISA Kit, IGBP1 ELISA Kit, ibp1 ELISA Kit, MGC147560 ELISA Kit, immunoglobulin binding protein 1 ELISA Kit, immunoglobulin (CD79A) binding protein 1 ELISA Kit, immunoglobulin (CD79A) binding protein 1 L homeolog ELISA Kit, IGBP1 ELISA Kit, Igbp1 ELISA Kit, igbp1 ELISA Kit, igbp1.L ELISA Kit

Hintergrund:

Gene Name: Immunoglobulin Binding Protein 1

Gene Aliases: IBP1, B-cell signal transduction molecule alpha 4, CD79a-binding protein 1, Protein phosphatase 2/4/6 regulatory subunit, Renal carcinoma antigen NY-REN-16