Indole 3 Acetic Acid CLIA Kit

Produktnummer ABIN5655327

Produktdetails Indole 3 Acetic Acid CLIA Kit

Target: Indole 3 Acetic Acid (IAA)

Reaktivität: Diverse Spezies

Nachweismethode: Chemiluminescent

Methodentyp: Competition ELISA

Detektionsbereich: 1.56 ng/mL - 400 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Indole 3 Acetic Acid (IAA). No significant cross-reactivity or interference between Indole 3 Acetic Acid (IAA) and analogues was observed.

Sensitivität: 0.63 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Indole 3 Acetic Acid (IAA). A competitive inhibition reaction is launched between biotin labeled Indole 3 Acetic Acid (IAA) and unlabeled Indole 3 Acetic Acid (IAA) (Standards or samples) with the pre-coated antibody specific to Indole 3 Acetic Acid (IAA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Indole 3 Acetic Acid (IAA) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Indole 3 Acetic Acid (IAA) level in the sample or standard.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Indole 3 Acetic Acid (IAA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Indole 3 Acetic Acid (IAA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: Indole 3 Acetic Acid (IAA)

Andere Bezeichnung: Indole 3 Acetic Acid (IAA Produkte)

Substanzklasse: Chemical

Hintergrund:

Gene Name: Indole 3 Acetic Acid

Gene Aliases: IA-A, Indolylacetic Acid, Indoleacetic Acid, Heteroauxin