XIAP ELISA Kit

Produktnummer ABIN5655359

Produktdetails XIAP ELISA Kit

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Inhibitor Of Apoptosis Protein, X-Linked (XIAP). No significant cross-reactivity or interference between Inhibitor Of Apoptosis Protein, X-Linked (XIAP) and analogues was observed.

Sensitivität: 0.056 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitor Of Apoptosis Protein, X-Linked (XIAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inhibitor Of Apoptosis Protein, X-Linked (XIAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibitor Of Apoptosis Protein, X-Linked (XIAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibitor Of Apoptosis Protein, X-Linked (XIAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Inhibitor Of Apoptosis Protein, X-Linked (XIAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Inhibitor Of Apoptosis Protein, X-Linked (XIAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu XIAP

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Andere Bezeichnung: Inhibitor Of Apoptosis Protein, X-Linked (XIAP Produkte)

Synonyme: API3 ELISA Kit, BIRC4 ELISA Kit, IAP-3 ELISA Kit, ILP1 ELISA Kit, MIHA ELISA Kit, XLP2 ELISA Kit, hIAP-3 ELISA Kit, hIAP3 ELISA Kit, api3 ELISA Kit, birc4 ELISA Kit, ilp1 ELISA Kit, miha ELISA Kit, xlp2 ELISA Kit, xxiap ELISA Kit, 1110015C02Rik ELISA Kit, Aipa ELISA Kit, Api3 ELISA Kit, Birc4 ELISA Kit, IAP3 ELISA Kit, ILP-1 ELISA Kit, mXIAP ELISA Kit, riap3 ELISA Kit, fb73d01 ELISA Kit, wu:fb73d01 ELISA Kit, zgc:91873 ELISA Kit, BIRC8 ELISA Kit, X-linked inhibitor of apoptosis ELISA Kit, X-linked inhibitor of apoptosis L homeolog ELISA Kit, XIAP ELISA Kit, xiap ELISA Kit, xiap.L ELISA Kit, Xiap ELISA Kit

Hintergrund:

Gene Name: Inhibitor Of Apoptosis Protein, X-Linked

Gene Aliases: BIRC4, API3, ILP1, MIHA, XLP2, Baculoviral IAP Repeat-Containing 4, E3 ubiquitin-protein ligase XIAP, IAP-like, Inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis protein

Gen-ID: 331

UniProt: P98170

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Transition Metal Ion Homeostasis