IGF2BP3 ELISA Kit

Produktnummer ABIN5655415

Produktdetails IGF2BP3 ELISA Kit

Target: IGF2BP3 (Insulin-Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). No significant cross-reactivity or interference between Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) and analogues was observed.

Sensitivität: 0.064 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu IGF2BP3

Target: IGF2BP3 (Insulin-Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3))

Andere Bezeichnung: Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3 Produkte)

Synonyme: CT98 ELISA Kit, IMP-3 ELISA Kit, IMP3 ELISA Kit, KOC1 ELISA Kit, VICKZ3 ELISA Kit, Ab2-255 ELISA Kit, RGD1306512 ELISA Kit, imp3 ELISA Kit, koc1 ELISA Kit, vera ELISA Kit, imp-3 ELISA Kit, vg1rbp ELISA Kit, vickz3 ELISA Kit, 2610101N11Rik ELISA Kit, AA522010 ELISA Kit, AL022933 ELISA Kit, AU045931 ELISA Kit, Koc13 ELISA Kit, Neilsen ELISA Kit, mimp3 ELISA Kit, dvr1rbp ELISA Kit, wu:fb79c12 ELISA Kit, wu:fe06h09 ELISA Kit, zVg1RBP ELISA Kit, B3 ELISA Kit, IGF2 mRNA-binding protein 3-B ELISA Kit, IMP ELISA Kit, IMP-3-B ELISA Kit, Vg1 RBP-B ELISA Kit, Vg1-RBP ELISA Kit, igf2bp3 ELISA Kit, igf2bp3-a ELISA Kit, igf2bp3-b ELISA Kit, IGF2 mRNA-binding protein 3-A ELISA Kit, IMP-3-A ELISA Kit, Vg1 RBP-A ELISA Kit, vera-A ELISA Kit, insulin like growth factor 2 mRNA binding protein 3 ELISA Kit, insulin-like growth factor 2 mRNA binding protein 3 ELISA Kit, insulin like growth factor 2 mRNA binding protein 3 S homeolog ELISA Kit, insulin like growth factor 2 mRNA binding protein 3 L homeolog ELISA Kit, IGF2BP3 ELISA Kit, Igf2bp3 ELISA Kit, igf2bp3 ELISA Kit, igf2bp3.S ELISA Kit, igf2bp3.L ELISA Kit

Hintergrund:

Gene Name: Insulin Like Growth Factor 2 mRNA Binding Protein 3

Gene Aliases: MP-3, IMP3, KOC1, VICKZ3, CT98, IGF II mRNA Binding Protein 3, Cancer/Testis Antigen 98, KH domain-containing protein overexpressed in cancer

Gen-ID: 10643

UniProt: O00425