Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

CD11b ELISA Kit

ITGAM Reaktivität: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5655495
  • Target Alle CD11b (ITGAM) ELISA Kits anzeigen
    CD11b (ITGAM) (Integrin alpha M (ITGAM))
    Reaktivität
    • 3
    • 3
    • 2
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    31.25 pg/mL - 2000 pg/mL
    Untere Nachweisgrenze
    31.25 pg/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Integrin Alpha M (ITGaM). No significant cross-reactivity or interference between Integrin Alpha M (ITGaM) and analogues was observed.
    Sensitivität
    13.3 pg/mL
    Featured
    Zu unserem meistverkauften ITGAM ELISA Kit
    Top Product
    Discover our top product ITGAM ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha M (ITGaM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Alpha M (ITGaM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha M (ITGaM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Alpha M (ITGaM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Alpha M (ITGaM) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Alpha M (ITGaM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle CD11b (ITGAM) ELISA Kits anzeigen
    CD11b (ITGAM) (Integrin alpha M (ITGAM))
    Andere Bezeichnung
    Integrin Alpha M (ITGAM Produkte)
    Synonyme
    CD11b/CD18 ELISA Kit, CR3 ELISA Kit, CR3A ELISA Kit, Cd11b ELISA Kit, F730045J24Rik ELISA Kit, Ly-40 ELISA Kit, MAC1 ELISA Kit, Mac-1 ELISA Kit, Mac-1a ELISA Kit, CD11B ELISA Kit, MAC-1 ELISA Kit, MAC1A ELISA Kit, MO1A ELISA Kit, SLEB6 ELISA Kit, C3bi ELISA Kit, integrin subunit alpha M ELISA Kit, integrin alpha M ELISA Kit, integrin, alpha M (complement component 3 receptor 3 subunit) ELISA Kit, integrin alpha-M ELISA Kit, ITGAM ELISA Kit, Itgam ELISA Kit, LOC100351865 ELISA Kit
    Hintergrund

    Gene Name: Integrin Alpha M

    Gene Aliases: CD11b, ITG-AM, CR3A, MAC-1, MAC1A, MO1A, Complement Component 3 Receptor 3 Subunit, Cell surface glycoprotein MAC-1 subunit alpha, Neutrophil adherence receptor

    Gen-ID
    3684
    UniProt
    P11215
    Pathways
    Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
Sie sind hier:
Kundenservice