CD47 ELISA Kit

Produktnummer ABIN5655499

Produktdetails CD47 ELISA Kit

Target: CD47

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Integrin Associated Protein (IAP). No significant cross-reactivity or interference between Integrin Associated Protein (IAP) and analogues was observed.

Sensitivität: 0.116 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Associated Protein (IAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Associated Protein (IAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Associated Protein (IAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Associated Protein (IAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Associated Protein (IAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Associated Protein (IAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu CD47

Target: CD47

Andere Bezeichnung: Integrin Associated Protein (CD47 Produkte)

Synonyme: IAP ELISA Kit, MER6 ELISA Kit, OA3 ELISA Kit, 9130415E20Rik ELISA Kit, AA407862 ELISA Kit, AI848868 ELISA Kit, AW108519 ELISA Kit, B430305P08Rik ELISA Kit, Itgp ELISA Kit, CD47/IAP ELISA Kit, alkaline phosphatase, intestinal ELISA Kit, CD47 molecule ELISA Kit, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) ELISA Kit, Cd47 molecule ELISA Kit, ALPI ELISA Kit, CD47 ELISA Kit, Cd47 ELISA Kit

Hintergrund:

Gene Name: Integrin Associated Protein

Gene Aliases: CD47, MER6, OA3, Antigen Identified By Monoclonal Antibody 1D8, Antigenic Surface Determinant Protein OA3, Rh-Related Antigen

Gen-ID: 961

UniProt: Q08722