Integrin beta 2 ELISA Kit

Produktnummer ABIN5655502

Produktdetails Integrin beta 2 ELISA Kit

Target: Integrin beta 2 (ITGB2)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Integrin Beta 2 (ITGb2). No significant cross-reactivity or interference between Integrin Beta 2 (ITGb2) and analogues was observed.

Sensitivität: 0.129 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Beta 2 (ITGb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Integrin Beta 2 (ITGb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Beta 2 (ITGb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Beta 2 (ITGb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Integrin Beta 2 (ITGb2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Integrin Beta 2 (ITGb2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Andere Bezeichnung: Integrin Beta 2 (ITGB2 Produkte)

Synonyme: CD18 ELISA Kit, LAD ELISA Kit, LCAMB ELISA Kit, LFA-1 ELISA Kit, MAC-1 ELISA Kit, MF17 ELISA Kit, MFI7 ELISA Kit, 2E6 ELISA Kit, AI528527 ELISA Kit, Cd18 ELISA Kit, Lfa1 ELISA Kit, ITGB2 ELISA Kit, itgb2 ELISA Kit, itgb2-a ELISA Kit, itgb2-b ELISA Kit, integrin subunit beta 2 ELISA Kit, integrin beta 2 ELISA Kit, integrin subunit beta 2 S homeolog ELISA Kit, ITGB2 ELISA Kit, Itgb2 ELISA Kit, itgb2.S ELISA Kit

Hintergrund:

Gene Name: Integrin Beta 2

Gene Aliases: CD18, ITG-B2, LAD, LFA1, MAC1, LCAMB, MF17, LFAb, Complement Component 3 Receptor 3 And 4 Subunit, Lymphocyte Function-Associated Antigen 1, Macrophage Antigen 1

Gen-ID: 3689

UniProt: P05107

Pathways: NF-kappaB Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation