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IFI35 ELISA Kit

IFI35 Reaktivität: Human Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5655617
  • Target Alle IFI35 ELISA Kits anzeigen
    IFI35 (Interferon-Induced Protein 35 (IFI35))
    Reaktivität
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    78.12 pg/mL - 5000 pg/mL
    Untere Nachweisgrenze
    78.12 pg/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Interferon Inducible Protein 35 (IFI35). No significant cross-reactivity or interference between Interferon Inducible Protein 35 (IFI35) and analogues was observed.
    Sensitivität
    31 pg/mL
    Top Product
    Discover our top product IFI35 ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Inducible Protein 35 (IFI35). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Inducible Protein 35 (IFI35). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Inducible Protein 35 (IFI35), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Inducible Protein 35 (IFI35) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Inducible Protein 35 (IFI35) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Inducible Protein 35 (IFI35) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle IFI35 ELISA Kits anzeigen
    IFI35 (Interferon-Induced Protein 35 (IFI35))
    Andere Bezeichnung
    Interferon Inducible Protein 35 (IFI35 Produkte)
    Synonyme
    IFI35 ELISA Kit, IFP35 ELISA Kit, 2010008K16Rik ELISA Kit, AW986054 ELISA Kit, ifi-35 ELISA Kit, interferon induced protein 35 ELISA Kit, interferon-induced protein 35 ELISA Kit, IFI35 ELISA Kit, ifi35 ELISA Kit, Ifi35 ELISA Kit
    Hintergrund

    Gene Name: Interferon Inducible Protein 35

    Gene Aliases: IFP35

    Gen-ID
    3430
    UniProt
    P80217
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