IDH2 ELISA Kit

Produktnummer ABIN5656123

Produktdetails IDH2 ELISA Kit

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Isocitrate Dehydrogenase 2, mitochondrial (IDH2). No significant cross-reactivity or interference between Isocitrate Dehydrogenase 2, mitochondrial (IDH2) and analogues was observed.

Sensitivität: 0.068 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Isocitrate Dehydrogenase 2, mitochondrial (IDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Isocitrate Dehydrogenase 2, mitochondrial (IDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Isocitrate Dehydrogenase 2, mitochondrial (IDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Isocitrate Dehydrogenase 2, mitochondrial (IDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Isocitrate Dehydrogenase 2, mitochondrial (IDH2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Isocitrate Dehydrogenase 2, mitochondrial (IDH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu IDH2

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Andere Bezeichnung: Isocitrate Dehydrogenase 2, mitochondrial (IDH2 Produkte)

Synonyme: F6P23.14 ELISA Kit, F6P23_14 ELISA Kit, IDH-II ELISA Kit, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 2 ELISA Kit, isocitrate dehydrogenase II ELISA Kit, isocitrate dehydrogenase subunit 2 ELISA Kit, D2HGA2 ELISA Kit, ICD-M ELISA Kit, IDH ELISA Kit, IDHM ELISA Kit, IDP ELISA Kit, IDPM ELISA Kit, mNADP-IDH ELISA Kit, E430004F23 ELISA Kit, IDPm ELISA Kit, Idh-2 ELISA Kit, wu:fb33c06 ELISA Kit, wu:fk31e05 ELISA Kit, wu:fq43b01 ELISA Kit, zgc:55485 ELISA Kit, idh2 ELISA Kit, IDH2 ELISA Kit, isocitrate dehydrogenase subunit 2 ELISA Kit, isocitrate dehydrogenase (NADP(+)) 2, mitochondrial ELISA Kit, isocitrate dehydrogenase 2 (NADP+), mitochondrial ELISA Kit, isocitrate dehydrogenase 2 (NADP+), mitochondrial S homeolog ELISA Kit, isocitrate dehydrogenase [NADP], mitochondrial ELISA Kit, IDH2 ELISA Kit, Idh2 ELISA Kit, idh2.S ELISA Kit, idh2 ELISA Kit, LOC100441867 ELISA Kit

Hintergrund:

Gene Name: Isocitrate Dehydrogenase 2, mitochondrial

Gene Aliases: IDH, IDP, ICD-M, IDHM, mNADP-IDH, Isocitrate dehydrogenase [NADP], mitochondrial, Oxalosuccinate decarboxylase

Gen-ID: 3418

UniProt: P48735

Pathways: Warburg Effekt