JAG1 ELISA Kit

Produktnummer ABIN5656126

Produktdetails JAG1 ELISA Kit

Target: JAG1 (Jagged 1 (JAG1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Jagged 1 Protein (JAG1). No significant cross-reactivity or interference between Jagged 1 Protein (JAG1) and analogues was observed.

Sensitivität: 6.0 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Jagged 1 Protein (JAG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Jagged 1 Protein (JAG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Jagged 1 Protein (JAG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Jagged 1 Protein (JAG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Jagged 1 Protein (JAG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Jagged 1 Protein (JAG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu JAG1

Target: JAG1 (Jagged 1 (JAG1))

Andere Bezeichnung: Jagged 1 Protein (JAG1 Produkte)

Synonyme: AGS ELISA Kit, AHD ELISA Kit, AWS ELISA Kit, CD339 ELISA Kit, HJ1 ELISA Kit, JAGL1 ELISA Kit, ABE2 ELISA Kit, Gsfabe2 ELISA Kit, Htu ELISA Kit, Ozz ELISA Kit, Ser-1 ELISA Kit, JAG1 ELISA Kit, jag3 ELISA Kit, cb243 ELISA Kit, jag1 ELISA Kit, sb:cb243 ELISA Kit, serrateC ELISA Kit, jagged1 ELISA Kit, serrate ELISA Kit, serrate-1 ELISA Kit, x-serrate-1 ELISA Kit, jagged 1 ELISA Kit, jagged 1b ELISA Kit, jagged 1a ELISA Kit, jagged 1 L homeolog ELISA Kit, JAG1 ELISA Kit, Jag1 ELISA Kit, jag1 ELISA Kit, jag1b ELISA Kit, jag1a ELISA Kit, jag1.L ELISA Kit

Hintergrund:

Gene Name: Jagged 1 Protein

Gene Aliases: CD339, AGS, AHD, AWS, HJ1, JAGL1, Alagille Syndrome

Gen-ID: 16449

UniProt: Q9QXX0

Pathways: Notch Signalweg, Stem Cell Maintenance