JKAMP ELISA Kit

Produktnummer ABIN5656133

Produktdetails JKAMP ELISA Kit

Target: JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Untere Nachweisgrenze: 1.56 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of JNK1/MAPK8 Associated Membrane Protein (JKAMP). No significant cross-reactivity or interference between JNK1/MAPK8 Associated Membrane Protein (JKAMP) and analogues was observed.

Sensitivität: 0.61 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to JNK1/MAPK8 Associated Membrane Protein (JKAMP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to JNK1/MAPK8 Associated Membrane Protein (JKAMP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain JNK1/MAPK8 Associated Membrane Protein (JKAMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of JNK1/MAPK8 Associated Membrane Protein (JKAMP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level JNK1/MAPK8 Associated Membrane Protein (JKAMP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level JNK1/MAPK8 Associated Membrane Protein (JKAMP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu JKAMP

Target: JKAMP (JNK1/MAPK8-Associated Membrane Protein (JKAMP))

Andere Bezeichnung: JNK1/MAPK8 Associated Membrane Protein (JKAMP Produkte)

Synonyme: C14orf100 ELISA Kit, HSPC213 ELISA Kit, HSPC327 ELISA Kit, JAMP ELISA Kit, 1200003C05Rik ELISA Kit, 2310047L11Rik ELISA Kit, Jamp ELISA Kit, RGD1308917 ELISA Kit, JNK1/MAPK8 associated membrane protein ELISA Kit, JNK1/MAPK8-associated membrane protein ELISA Kit, JKAMP ELISA Kit, Jkamp ELISA Kit

Hintergrund:

Gene Name: JNK1/MAPK8 Associated Membrane Protein

Gene Aliases: JAMP, C14orf100, Jun N-Terminal Kinase 1-Associated Membrane Protein, Medulloblastoma antigen MU-MB-50.4