JUP ELISA Kit

Produktnummer ABIN5656136

Produktdetails JUP ELISA Kit

Target: JUP (Junction Plakoglobin (JUP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Junction Plakoglobin (JUP). No significant cross-reactivity or interference between Junction Plakoglobin (JUP) and analogues was observed.

Sensitivität: 0.056 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Junction Plakoglobin (JUP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Junction Plakoglobin (JUP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Junction Plakoglobin (JUP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Junction Plakoglobin (JUP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Junction Plakoglobin (JUP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Junction Plakoglobin (JUP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu JUP

Target: JUP (Junction Plakoglobin (JUP))

Andere Bezeichnung: Junction Plakoglobin (JUP Produkte)

Synonyme: ARVD12 ELISA Kit, CTNNG ELISA Kit, DP3 ELISA Kit, DPIII ELISA Kit, PDGB ELISA Kit, PKGB ELISA Kit, arvd12 ELISA Kit, ctnng ELISA Kit, dp3 ELISA Kit, dpiii ELISA Kit, pdgb ELISA Kit, pkgb ELISA Kit, plak ELISA Kit, D930025P04Rik ELISA Kit, PG ELISA Kit, cb80 ELISA Kit, danPG ELISA Kit, fb11b06 ELISA Kit, jup ELISA Kit, wu:fb11b06 ELISA Kit, junction plakoglobin ELISA Kit, junction plakoglobin L homeolog ELISA Kit, Junction plakoglobin ELISA Kit, junction plakoglobin a ELISA Kit, junction plakoglobin S homeolog ELISA Kit, JUP ELISA Kit, Jup ELISA Kit, jup ELISA Kit, jup.L ELISA Kit, Tsp_06656 ELISA Kit, plak ELISA Kit, jupa ELISA Kit, jup.S ELISA Kit

Hintergrund:

Gene Name: Junction Plakoglobin

Gene Aliases: DP3, gCat, G-Cat, CTNNG, DPIII, PDGB, PKGB, Gamma Catenin, Desmoplakin III, Desmoplakin-3

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location