Kallikrein 14 ELISA Kit

Produktnummer ABIN5656151

Produktdetails Kallikrein 14 ELISA Kit

Target: Kallikrein 14 (KLK14)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 123.46 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 123.46 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Kallikrein 14 (KLK14). No significant cross-reactivity or interference between Kallikrein 14 (KLK14) and analogues was observed.

Sensitivität: 43.2 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Kallikrein 14 (KLK14) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Kallikrein 14 (KLK14) and unlabeled Kallikrein 14 (KLK14) (Standards or samples) with the pre-coated antibody specific to Kallikrein 14 (KLK14). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Kallikrein 14 (KLK14) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Kallikrein 14 (KLK14) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kallikrein 14 (KLK14) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kallikrein 14 (KLK14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Kallikrein 14

Target: Kallikrein 14 (KLK14)

Andere Bezeichnung: Kallikrein 14 (KLK14 Produkte)

Synonyme: GK14 ELISA Kit, KLK-L6 ELISA Kit, kallikrein related-peptidase 14 ELISA Kit, kallikrein related peptidase 14 ELISA Kit, Klk14 ELISA Kit, KLK14 ELISA Kit

Hintergrund:

Gene Name: Kallikrein 14

Gene Aliases: KLK-L6, Kallikrein-like protein 6

Gen-ID: 43847

UniProt: Q9P0G3

Pathways: Komplementsystem, Regulation of G-Protein Coupled Receptor Protein Signaling