KEAP1 ELISA Kit

Produktnummer ABIN5656176

Produktdetails KEAP1 ELISA Kit

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Kelch Like ECH Associated Protein 1 (KEAP1). No significant cross-reactivity or interference between Kelch Like ECH Associated Protein 1 (KEAP1) and analogues was observed.

Sensitivität: 0.055 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kelch Like ECH Associated Protein 1 (KEAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kelch Like ECH Associated Protein 1 (KEAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kelch Like ECH Associated Protein 1 (KEAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kelch Like ECH Associated Protein 1 (KEAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kelch Like ECH Associated Protein 1 (KEAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kelch Like ECH Associated Protein 1 (KEAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu KEAP1

Target: KEAP1 (Kelch-Like ECH-Associated Protein 1 (KEAP1))

Andere Bezeichnung: Kelch Like ECH Associated Protein 1 (KEAP1 Produkte)

Synonyme: INrf2 ELISA Kit, KLHL19 ELISA Kit, INRF2 ELISA Kit, mKIAA0132 ELISA Kit, Inrf2 ELISA Kit, fb36f11 ELISA Kit, keap1 ELISA Kit, wu:fb36f11 ELISA Kit, KEAP1 ELISA Kit, klhl19 ELISA Kit, zgc:171654 ELISA Kit, kelch like ECH associated protein 1 ELISA Kit, kelch-like ECH-associated protein 1 ELISA Kit, Kelch-like ECH-associated protein 1 ELISA Kit, kelch-like ECH-associated protein 1a ELISA Kit, kelch like ECH associated protein 1 L homeolog ELISA Kit, kelch-like ECH-associated protein 1b ELISA Kit, KEAP1 ELISA Kit, Keap1 ELISA Kit, keap1a ELISA Kit, keap1 ELISA Kit, keap1.L ELISA Kit, keap1b ELISA Kit

Hintergrund:

Gene Name: Kelch Like ECH Associated Protein 1

Gene Aliases: INrf2, KLHL19, Cytosolic inhibitor of Nrf2, Kelch-like protein 19

Gen-ID: 50868

UniProt: Q9Z2X8

Pathways: Maintenance of Protein Location