KRT15 ELISA Kit

Produktnummer ABIN5656183

Produktdetails KRT15 ELISA Kit

Target: KRT15 (Keratin 15 (KRT15))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Keratin 15 (KRT15). No significant cross-reactivity or interference between Keratin 15 (KRT15) and analogues was observed.

Sensitivität: 13.7 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 15 (KRT15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 15 (KRT15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 15 (KRT15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 15 (KRT15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 15 (KRT15) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 15 (KRT15) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu KRT15

Target: KRT15 (Keratin 15 (KRT15))

Andere Bezeichnung: Keratin 15 (KRT15 Produkte)

Synonyme: xlk2 ELISA Kit, CK15 ELISA Kit, K15 ELISA Kit, K1CO ELISA Kit, AI528832 ELISA Kit, Krt1-15 ELISA Kit, Ka15 ELISA Kit, wu:fa91c06 ELISA Kit, wu:fa95d04 ELISA Kit, zgc:55334 ELISA Kit, keratin 15, type I L homeolog ELISA Kit, keratin 15, type I ELISA Kit, keratin 15 ELISA Kit, krt15.L ELISA Kit, krt15 ELISA Kit, KRT15 ELISA Kit, Krt15 ELISA Kit

Hintergrund:

Gene Name: Keratin 15

Gene Aliases: CK15, K15, CK15, K1CO, KRTB, Cytokeratin 15, Keratin-15,Basic, Keratin-15,Beta, Keratin, type I cytoskeletal 15

Gen-ID: 3866

UniProt: P19012