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LACTB ELISA Kit

LACTB Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
Produktnummer ABIN5656269
  • Target Alle LACTB ELISA Kits anzeigen
    LACTB (Lactamase, beta (LACTB))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Lactamase Beta (LACTb). No significant cross-reactivity or interference between Lactamase Beta (LACTb) and analogues was observed.
    Sensitivität
    0.122 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lactamase Beta (LACTb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lactamase Beta (LACTb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lactamase Beta (LACTb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lactamase Beta (LACTb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactamase Beta (LACTb) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactamase Beta (LACTb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle LACTB ELISA Kits anzeigen
    LACTB (Lactamase, beta (LACTB))
    Andere Bezeichnung
    Lactamase Beta (LACTB Produkte)
    Synonyme
    BA2507 ELISA Kit, PSPTO2834 ELISA Kit, PSPTO3594 ELISA Kit, G24 ELISA Kit, MRPL56 ELISA Kit, LACT-1 ELISA Kit, Lact1 ELISA Kit, Mrpl56 ELISA Kit, zgc:110419 ELISA Kit, beta-lactamase ELISA Kit, class C beta-lactamase AmpC ELISA Kit, metal-dependent hydrolase ELISA Kit, lactamase beta ELISA Kit, Beta-lactamase ELISA Kit, lactamase, beta ELISA Kit, metallo-beta-lactamase domain containing 2 ELISA Kit, Beta lactamase ELISA Kit, bla1 ELISA Kit, ampC ELISA Kit, PSPTO_2834 ELISA Kit, GSU1378 ELISA Kit, MCA2220 ELISA Kit, CJE0344 ELISA Kit, LACTB ELISA Kit, Rmar_1357 ELISA Kit, MGYG_07395 ELISA Kit, ML5_3452 ELISA Kit, bla ELISA Kit, Lactb ELISA Kit, lactb ELISA Kit, MBLAC2 ELISA Kit, blaKPC-2 ELISA Kit
    Hintergrund

    Gene Name: Lactamase Beta

    Gene Aliases: G24, MRPL56, Mitochondrial Ribosomal Protein L56, Serine beta-lactamase-like protein LACTB, mitochondrial

    Gen-ID
    114294
    UniProt
    P83111
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