Laminin gamma 1 ELISA Kit

Produktnummer ABIN5656319

Produktdetails Laminin gamma 1 ELISA Kit

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.12 ng/mL - 200 ng/mL

Untere Nachweisgrenze: 3.12 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Laminin Gamma 1 (LAMC1). No significant cross-reactivity or interference between Laminin Gamma 1 (LAMC1) and analogues was observed.

Sensitivität: 1.32 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Gamma 1 (LAMC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Gamma 1 (LAMC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Gamma 1 (LAMC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Gamma 1 (LAMC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Laminin Gamma 1 (LAMC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Laminin Gamma 1 (LAMC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Laminin gamma 1

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Andere Bezeichnung: Laminin Gamma 1 (LAMC1 Produkte)

Synonyme: lamb2 ELISA Kit, Lamb2 ELISA Kit, B2e ELISA Kit, sly ELISA Kit, LAMB2 ELISA Kit, putative laminin gamma-1 chain ELISA Kit, laminin subunit gamma 1 ELISA Kit, laminin, gamma 1 ELISA Kit, Smp_163810 ELISA Kit, lamc1 ELISA Kit, Lamc1 ELISA Kit, LAMC1 ELISA Kit

Hintergrund:

Gene Name: Laminin Gamma 1

Gene Aliases: LAM-C1, LAMB2, S-laminin subunit gamma, Laminin B2 chain

Gen-ID: 3915

UniProt: P11047