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LEFTY1 CLIA Kit

LEFTY1 Reaktivität: Human Chemiluminescent Sandwich ELISA 156.25 pg/mL - 10000 pg/mL Plasma, Serum, Tissue Homogenate
Produktnummer ABIN5656345
  • Target Alle LEFTY1 CLIA Kits anzeigen
    LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))
    Reaktivität
    • 4
    • 3
    • 3
    Human
    Nachweismethode
    Chemiluminescent
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    156.25 pg/mL - 10000 pg/mL
    Untere Nachweisgrenze
    156.25 pg/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 1 (LEFTY1). No significant cross-reactivity or interference between Left/Right Determination Factor 1 (LEFTY1) and analogues was observed.
    Sensitivität
    5.31 pg/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    2 - 3 h
    Plattentyp
    Pre-coated
    Protokoll
    The microplate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Left/Right Determination Factor 1 (LEFTY1) level in the sample or standard.,
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle LEFTY1 CLIA Kits anzeigen
    LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))
    Andere Bezeichnung
    Left/Right Determination Factor 1 (LEFTY1 Produkte)
    Synonyme
    atv CLIA Kit, cb73 CLIA Kit, antivin CLIA Kit, ik:tdsubc_2b12 CLIA Kit, xx:tdsubc_2b12 CLIA Kit, LEFTY1 CLIA Kit, AI450052 CLIA Kit, Ebaf CLIA Kit, Leftb CLIA Kit, Stra3 CLIA Kit, Tgfb4 CLIA Kit, lefty CLIA Kit, lefty-1 CLIA Kit, RGD1561867 CLIA Kit, LEFTB CLIA Kit, LEFTYB CLIA Kit, lefty1 CLIA Kit, signaling molecule lefty1 CLIA Kit, left-right determination factor 1 CLIA Kit, left right determination factor 1 CLIA Kit, lft1 CLIA Kit, lefty1 CLIA Kit, LOC699924 CLIA Kit, LEFTY1 CLIA Kit, LOC100408003 CLIA Kit, LOC100597378 CLIA Kit, Lefty1 CLIA Kit
    Hintergrund

    Gene Name: Left/Right Determination Factor 1

    Gene Aliases: LEFTY-B, LEFTYB, LEFT-B, LEFTB, Left Right Determination Factor B

    Gen-ID
    10637
    UniProt
    O75610
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