LEFTY1 CLIA Kit

Produktnummer ABIN5656349

Produktdetails LEFTY1 CLIA Kit

Target: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Reaktivität: Ratte

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 156.25 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156.25 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Left/Right Determination Factor 1 (LEFTY1). No significant cross-reactivity or interference between Left/Right Determination Factor 1 (LEFTY1) and analogues was observed.

Sensitivität: 4.77 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Left/Right Determination Factor 1 (LEFTY1) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: LEFTY1 (Left-Right Determination Factor 1 (LEFTY1))

Andere Bezeichnung: Left/Right Determination Factor 1 (LEFTY1 Produkte)

Synonyme: atv CLIA Kit, cb73 CLIA Kit, antivin CLIA Kit, ik:tdsubc_2b12 CLIA Kit, xx:tdsubc_2b12 CLIA Kit, LEFTY1 CLIA Kit, AI450052 CLIA Kit, Ebaf CLIA Kit, Leftb CLIA Kit, Stra3 CLIA Kit, Tgfb4 CLIA Kit, lefty CLIA Kit, lefty-1 CLIA Kit, RGD1561867 CLIA Kit, LEFTB CLIA Kit, LEFTYB CLIA Kit, lefty1 CLIA Kit, signaling molecule lefty1 CLIA Kit, left-right determination factor 1 CLIA Kit, left right determination factor 1 CLIA Kit, lft1 CLIA Kit, lefty1 CLIA Kit, LOC699924 CLIA Kit, LEFTY1 CLIA Kit, LOC100408003 CLIA Kit, LOC100597378 CLIA Kit, Lefty1 CLIA Kit

Hintergrund:

Gene Name: Left/Right Determination Factor 1

Gene Aliases: LEFTY-B, LEFTYB, LEFT-B, LEFTB, Left Right Determination Factor B

Gen-ID: 498299

UniProt: D4A670