LRRFIP1 ELISA Kit

Produktnummer ABIN5656391

Produktdetails LRRFIP1 ELISA Kit

Target: LRRFIP1 (Leucine Rich Repeat (In FLII) Interacting Protein 1 (LRRFIP1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1). No significant cross-reactivity or interference between Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) and analogues was observed.

Sensitivität: 0.127 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu LRRFIP1

Target: LRRFIP1 (Leucine Rich Repeat (In FLII) Interacting Protein 1 (LRRFIP1))

Andere Bezeichnung: Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1 Produkte)

Synonyme: hm:zeh1367 ELISA Kit, lrrfip1 ELISA Kit, wu:fc06b04 ELISA Kit, wu:fi15c07 ELISA Kit, zeh1367 ELISA Kit, LRRFIP1 ELISA Kit, AU024550 ELISA Kit, Fliiap1 ELISA Kit, FLAP-1 ELISA Kit, FLAP1 ELISA Kit, FLIIAP1 ELISA Kit, GCF-2 ELISA Kit, GCF2 ELISA Kit, HUFI-1 ELISA Kit, TRIP ELISA Kit, leucine rich repeat (in FLII) interacting protein 1a ELISA Kit, LRR binding FLII interacting protein 1 ELISA Kit, leucine rich repeat (in FLII) interacting protein 1 ELISA Kit, lrrfip1a ELISA Kit, LRRFIP1 ELISA Kit, Lrrfip1 ELISA Kit

Hintergrund:

Gene Name: Leucine Rich Repeat In FLII Interacting Protein 1

Gene Aliases: GCF2, FLAP-1, FLIIAP1, GCF-2, HUFI-1, TRIP, GC-Binding Factor 2, TAR RNA-interacting protein

Gen-ID: 9208

UniProt: Q32MZ4