Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

LIM Domain Binding 1 Protein ELISA Kit

LDB1 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Produktnummer ABIN5656421
  • Target Alle LIM Domain Binding 1 Protein (LDB1) ELISA Kits anzeigen
    LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))
    Reaktivität
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of LIM Domain Binding Protein 1 (LDB1). No significant cross-reactivity or interference between LIM Domain Binding Protein 1 (LDB1) and analogues was observed.
    Sensitivität
    0.061 ng/mL
    Featured
    Zu unserem meistverkauften LDB1 ELISA Kit
    Top Product
    Discover our top product LDB1 ELISA Kit
  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIM Domain Binding Protein 1 (LDB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to LIM Domain Binding Protein 1 (LDB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIM Domain Binding Protein 1 (LDB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LIM Domain Binding Protein 1 (LDB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LIM Domain Binding Protein 1 (LDB1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LIM Domain Binding Protein 1 (LDB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle LIM Domain Binding 1 Protein (LDB1) ELISA Kits anzeigen
    LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))
    Andere Bezeichnung
    LIM Domain Binding Protein 1 (LDB1 Produkte)
    Synonyme
    CLIM2 ELISA Kit, NLI ELISA Kit, chi ELISA Kit, clim2 ELISA Kit, nli ELISA Kit, xldb1 ELISA Kit, ldb1 ELISA Kit, LDB1 ELISA Kit, ldb4 ELISA Kit, ldb4b ELISA Kit, LIM domain binding 1 ELISA Kit, LIM domain binding 1 L homeolog ELISA Kit, LIM-domain binding 1b ELISA Kit, LIM domain binding protein 1 ELISA Kit, LIM domain binding 1a ELISA Kit, Ldb1 ELISA Kit, LDB1 ELISA Kit, ldb1.L ELISA Kit, ldb1b ELISA Kit, xldb1 ELISA Kit, ldb1a ELISA Kit
    Hintergrund

    Gene Name: LIM Domain Binding Protein 1

    Gene Aliases: CLIM2, NLI, Carboxy Terminal LIM Domain Protein 2, LIM domain-binding factor CLIM2, Nuclear LIM interactor

    Gen-ID
    8861
    UniProt
    Q86U70
    Pathways
    Stem Cell Maintenance, Chromatin Binding
Sie sind hier:
Kundenservice