LBP ELISA Kit
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- Target Alle LBP ELISA Kits anzeigen
- LBP (Lipopolysaccharide Binding Protein (LBP))
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Reaktivität
- Rind (Kuh)
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.625 ng/mL - 40 ng/mL
- Untere Nachweisgrenze
- 0.625 ng/mL
- Applikation
- ELISA
- Proben
- Plasma, Serum
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Lipopolysaccharide Binding Protein (LBP). No significant cross-reactivity or interference between Lipopolysaccharide Binding Protein (LBP) and analogues was observed.
- Sensitivität
- 0.288 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipopolysaccharide Binding Protein (LBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipopolysaccharide Binding Protein (LBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipopolysaccharide Binding Protein (LBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipopolysaccharide Binding Protein (LBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipopolysaccharide Binding Protein (LBP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipopolysaccharide Binding Protein (LBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle LBP ELISA Kits anzeigen
- LBP (Lipopolysaccharide Binding Protein (LBP))
- Andere Bezeichnung
- Lipopolysaccharide Binding Protein (LBP Produkte)
- Synonyme
- BPIFD2 ELISA Kit, Bpifd2 ELISA Kit, Ly88 ELISA Kit, LPSBP ELISA Kit, LBP ELISA Kit, lipopolysaccharide binding protein ELISA Kit, lipopolysaccharide-binding protein ELISA Kit, LBP ELISA Kit, Lbp ELISA Kit, LOC100472839 ELISA Kit
- Hintergrund
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Gene Name: Lipopolysaccharide Binding Protein
Gene Aliases: LPS-Binding Protein
- UniProt
- Q2TBI0
- Pathways
- TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process
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