LRP5 ELISA Kit

Produktnummer ABIN5656496

Produktdetails LRP5 ELISA Kit

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Low Density Lipoprotein Receptor Related Protein 5 (LRP5). No significant cross-reactivity or interference between Low Density Lipoprotein Receptor Related Protein 5 (LRP5) and analogues was observed.

Sensitivität: 0.3 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Related Protein 5 (LRP5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Related Protein 5 (LRP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Density Lipoprotein Receptor Related Protein 5 (LRP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Low Density Lipoprotein Receptor Related Protein 5 (LRP5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 5 (LRP5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Related Protein 5 (LRP5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu LRP5

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Andere Bezeichnung: Low Density Lipoprotein Receptor Related Protein 5 (LRP5 Produkte)

Synonyme: Xlrp5 ELISA Kit, bmnd1 ELISA Kit, evr1 ELISA Kit, evr4 ELISA Kit, hbm ELISA Kit, lr3 ELISA Kit, lrp5-A ELISA Kit, lrp7 ELISA Kit, oppg ELISA Kit, ops ELISA Kit, opta1 ELISA Kit, vbch2 ELISA Kit, LRP5 ELISA Kit, LRP6 ELISA Kit, BMND1 ELISA Kit, EVR1 ELISA Kit, EVR4 ELISA Kit, HBM ELISA Kit, LR3 ELISA Kit, LRP7 ELISA Kit, OPPG ELISA Kit, OPS ELISA Kit, OPTA1 ELISA Kit, VBCH2 ELISA Kit, mKIAA4142 ELISA Kit, low-density lipoprotein receptor-related protein 5 ELISA Kit, LDL receptor related protein 5 ELISA Kit, LDL receptor related protein 5 L homeolog ELISA Kit, low density lipoprotein receptor-related protein 5 ELISA Kit, LDL receptor related protein 5 like ELISA Kit, LOC100147353 ELISA Kit, LRP5 ELISA Kit, lrp5.L ELISA Kit, lrp5 ELISA Kit, Lrp5 ELISA Kit, LRP5L ELISA Kit

Hintergrund:

Gene Name: Low Density Lipoprotein Receptor Related Protein 5

Gene Aliases: HBM, BMND1, EVR1, EVR4, LR3, LRP7, OPPG, OPS, OPTA1, VBCH2, Osteoporosis Pseudoglioma Syndrome, Exudative Vitreoretinopathy 1

Gen-ID: 293649

UniProt: B2RYI1

Pathways: WNT Signalweg, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation