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MCAM CLIA Kit

MCAM Reaktivität: Maus Chemiluminescent Sandwich ELISA 0.156 ng/mL - 40 ng/mL Plasma, Serum
Produktnummer ABIN5656812
  • Target Alle MCAM CLIA Kits anzeigen
    MCAM (Melanoma Cell Adhesion Molecule (MCAM))
    Reaktivität
    • 7
    • 2
    • 1
    Maus
    Nachweismethode
    Chemiluminescent
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 40 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Melanoma Cell Adhesion Molecule (MCAM). No significant cross-reactivity or interference between Melanoma Cell Adhesion Molecule (MCAM) and analogues was observed.
    Sensitivität
    0.03 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    2 - 3 h
    Plattentyp
    Pre-coated
    Protokoll
    The microplate provided in this kit has been pre-coated with an antibody specific to Melanoma Cell Adhesion Molecule (MCAM). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Melanoma Cell Adhesion Molecule (MCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Melanoma Cell Adhesion Molecule (MCAM) level in the sample or standard.,
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanoma Cell Adhesion Molecule (MCAM) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanoma Cell Adhesion Molecule (MCAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle MCAM CLIA Kits anzeigen
    MCAM (Melanoma Cell Adhesion Molecule (MCAM))
    Andere Bezeichnung
    Melanoma Cell Adhesion Molecule (MCAM Produkte)
    Synonyme
    CD146 CLIA Kit, MUC18 CLIA Kit, Muc18 CLIA Kit, 1-gicerin CLIA Kit, AV025631 CLIA Kit, CD149 CLIA Kit, s-endo CLIA Kit, s-gicerin CLIA Kit, cd146 CLIA Kit, gicerin CLIA Kit, id:ibd3512 CLIA Kit, mcam CLIA Kit, MCAM CLIA Kit, melanoma cell adhesion molecule CLIA Kit, melanoma cell adhesion molecule b CLIA Kit, MCAM CLIA Kit, Mcam CLIA Kit, mcamb CLIA Kit
    Hintergrund

    Gene Name: Melanoma Cell Adhesion Molecule

    Gene Aliases: CD146, MUC18, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, S-endo 1 endothelial-associated antigen

    Gen-ID
    84004
    UniProt
    Q8R2Y2
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