MIA ELISA Kit

Produktnummer ABIN5656816

Produktdetails MIA ELISA Kit

Target: MIA (Melanoma Inhibitory Activity (MIA))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Melanoma Inhibitory Activity Protein 1 (MIA1). No significant cross-reactivity or interference between Melanoma Inhibitory Activity Protein 1 (MIA1) and analogues was observed.

Sensitivität: 37 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Inhibitory Activity Protein 1 (MIA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Melanoma Inhibitory Activity Protein 1 (MIA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanoma Inhibitory Activity Protein 1 (MIA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanoma Inhibitory Activity Protein 1 (MIA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Melanoma Inhibitory Activity Protein 1 (MIA1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Melanoma Inhibitory Activity Protein 1 (MIA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Andere Bezeichnung: Melanoma Inhibitory Activity Protein 1 (MIA Produkte)

Synonyme: CD-RAP ELISA Kit, Mia1 ELISA Kit, Cdrap ELISA Kit, zgc:194157 ELISA Kit, mia ELISA Kit, CDRAP ELISA Kit, Fdp ELISA Kit, MIA ELISA Kit, MIAL ELISA Kit, melanoma inhibitory activity ELISA Kit, otoraplin ELISA Kit, cilia and flagella associated protein 100 ELISA Kit, MIA ELISA Kit, Mia ELISA Kit, mia ELISA Kit, Otor ELISA Kit, CFAP100 ELISA Kit

Hintergrund:

Gene Name: Melanoma Inhibitory Activity Protein 1

Gene Aliases: CD-RAP, Melanoma-derived growth regulatory protein

Gen-ID: 12587

UniProt: Q61865