MAPRE1 ELISA Kit

Produktnummer ABIN5656894

Produktdetails MAPRE1 ELISA Kit

Target: MAPRE1 (Microtubule-Associated Protein, RP/EB Family, Member 1 (MAPRE1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1). No significant cross-reactivity or interference between Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) and analogues was observed.

Sensitivität: 0.058 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MAPRE1

Target: MAPRE1 (Microtubule-Associated Protein, RP/EB Family, Member 1 (MAPRE1))

Andere Bezeichnung: Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1 Produkte)

Synonyme: EB1 ELISA Kit, 5530600P05Rik ELISA Kit, AI462499 ELISA Kit, AI504412 ELISA Kit, AW260097 ELISA Kit, BIM1p ELISA Kit, D2Ertd459e ELISA Kit, Eb1 ELISA Kit, MAPRE1 ELISA Kit, mapre1l ELISA Kit, wu:fi33c06 ELISA Kit, zgc:55428 ELISA Kit, zgc:77807 ELISA Kit, mapre1 ELISA Kit, wu:fa01e12 ELISA Kit, wu:fc23e11 ELISA Kit, zgc:85755 ELISA Kit, MGC52508 ELISA Kit, eb1 ELISA Kit, xEB1B ELISA Kit, Mapre3 ELISA Kit, microtubule associated protein RP/EB family member 1 ELISA Kit, microtubule-associated protein, RP/EB family, member 1 ELISA Kit, microtubule-associated protein, RP/EB family, member 1a ELISA Kit, microtubule-associated protein, RP/EB family, member 1b ELISA Kit, microtubule associated protein RP/EB family member 1 L homeolog ELISA Kit, microtubule associated protein RP/EB family member 1 S homeolog ELISA Kit, MAPRE1 ELISA Kit, Mapre1 ELISA Kit, mapre1a ELISA Kit, mapre1b ELISA Kit, mapre1.L ELISA Kit, mapre1.S ELISA Kit

Hintergrund:

Gene Name: Microtubule Associated Protein RP/EB Family, Member 1

Gene Aliases: EB1, Adenomatous Polyposis Coli-Binding protein, APC-binding protein EB1, End-binding protein 1

Gen-ID: 22919

UniProt: Q15691

Pathways: M Phase