MCM3 ELISA Kit

Produktnummer ABIN5656914

Produktdetails MCM3 ELISA Kit

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Minichromosome Maintenance Deficient 3 (MCM3). No significant cross-reactivity or interference between Minichromosome Maintenance Deficient 3 (MCM3) and analogues was observed.

Sensitivität: 0.058 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Minichromosome Maintenance Deficient 3 (MCM3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Minichromosome Maintenance Deficient 3 (MCM3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Minichromosome Maintenance Deficient 3 (MCM3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Minichromosome Maintenance Deficient 3 (MCM3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Minichromosome Maintenance Deficient 3 (MCM3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Minichromosome Maintenance Deficient 3 (MCM3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MCM3

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Andere Bezeichnung: Minichromosome Maintenance Deficient 3 (MCM3 Produkte)

Synonyme: HCC5 ELISA Kit, P1-MCM3 ELISA Kit, P1.h ELISA Kit, RLFB ELISA Kit, AL033361 ELISA Kit, C80350 ELISA Kit, Mcmd ELISA Kit, P1 ELISA Kit, p1.m ELISA Kit, cb32 ELISA Kit, chunp6867 ELISA Kit, wu:fa26g03 ELISA Kit, CG4206 ELISA Kit, DmMCM3 ELISA Kit, DmMcm3 ELISA Kit, DmeMCM3 ELISA Kit, Dmel\\CG4206 ELISA Kit, MCM3 ELISA Kit, McM3 ELISA Kit, Mcp-PCR2 ELISA Kit, PCR2 ELISA Kit, dMCM3 ELISA Kit, xmcm3 ELISA Kit, minichromosome maintenance complex component 3 ELISA Kit, MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) ELISA Kit, Minichromosome maintenance 3 ELISA Kit, minichromosome maintenance complex component 3 L homeolog ELISA Kit, MCM complex subunit Mcm3 ELISA Kit, MCM3 ELISA Kit, mcm3 ELISA Kit, Mcm3 ELISA Kit, mcm3.L ELISA Kit

Hintergrund:

Gene Name: Minichromosome Maintenance Deficient 3

Gene Aliases: HCC5, P1-MCM3, P1.h, RLFB, RLF subunit beta, DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1

Gen-ID: 4172

UniProt: P25205

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA