MAX ELISA Kit

Produktnummer ABIN5657025

Produktdetails MAX ELISA Kit

Target: MAX (MYC Associated Factor X (MAX))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of MYC Associated Factor X (MAX). No significant cross-reactivity or interference between MYC Associated Factor X (MAX) and analogues was observed.

Sensitivität: 0.059 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MYC Associated Factor X (MAX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MYC Associated Factor X (MAX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MYC Associated Factor X (MAX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MYC Associated Factor X (MAX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MYC Associated Factor X (MAX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MYC Associated Factor X (MAX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MAX

Target: MAX (MYC Associated Factor X (MAX))

Andere Bezeichnung: MYC Associated Factor X (MAX Produkte)

Synonyme: CG9648 ELISA Kit, Dmel\\CG9648 ELISA Kit, MAX ELISA Kit, bHLHd9 ELISA Kit, dMax ELISA Kit, dmax ELISA Kit, max ELISA Kit, bhlhd4 ELISA Kit, bhlhd5 ELISA Kit, bhlhd6 ELISA Kit, bhlhd7 ELISA Kit, bhlhd8 ELISA Kit, xmax2 ELISA Kit, xmax4 ELISA Kit, xmax ELISA Kit, AA960152 ELISA Kit, AI875693 ELISA Kit, bHLHd4 ELISA Kit, bHLHd5 ELISA Kit, bHLHd6 ELISA Kit, bHLHd7 ELISA Kit, bHLHd8 ELISA Kit, fa92b04 ELISA Kit, fb52a04 ELISA Kit, fb95h11 ELISA Kit, wu:fa92b04 ELISA Kit, wu:fb52a04 ELISA Kit, wu:fb95h11 ELISA Kit, zmax ELISA Kit, CG9648 gene product from transcript CG9648-RD ELISA Kit, MYC associated factor X L homeolog ELISA Kit, MYC associated factor X ELISA Kit, Max protein ELISA Kit, MYC associated factor X S homeolog ELISA Kit, myc associated factor X ELISA Kit, Max ELISA Kit, max.L ELISA Kit, MAX ELISA Kit, max ELISA Kit, max.S ELISA Kit

Hintergrund:

Gene Name: MYC Associated Factor X

Gene Aliases: BHLHD4, Class D basic helix-loop-helix protein 4

Gen-ID: 4149

UniProt: P61244

Pathways: Mitotic G1-G1/S Phases