MYD88 ELISA Kit

Produktnummer ABIN5657045

Produktdetails MYD88 ELISA Kit

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Myeloid Differentiation Factor 88 (MyD88). No significant cross-reactivity or interference between Myeloid Differentiation Factor 88 (MyD88) and analogues was observed.

Sensitivität: 0.055 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Differentiation Factor 88 (MyD88). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myeloid Differentiation Factor 88 (MyD88). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Differentiation Factor 88 (MyD88), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloid Differentiation Factor 88 (MyD88) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Differentiation Factor 88 (MyD88) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Differentiation Factor 88 (MyD88) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MYD88

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Andere Bezeichnung: Myeloid Differentiation Factor 88 (MYD88 Produkte)

Synonyme: MYD88D ELISA Kit, XMyD88 ELISA Kit, myd88d ELISA Kit, MGC84928 ELISA Kit, CG2078 ELISA Kit, DMMYD88 ELISA Kit, DmMyD88 ELISA Kit, DmMyd88 ELISA Kit, Dmel\\CG2078 ELISA Kit, EP(2)2535 ELISA Kit, Kra ELISA Kit, LD20892 ELISA Kit, MYD88 ELISA Kit, MyD88 ELISA Kit, Myd88F ELISA Kit, dMyD88 ELISA Kit, dMyd88 ELISA Kit, kra ELISA Kit, myd88 ELISA Kit, zgc:103541 ELISA Kit, GB12344 ELISA Kit, NV10640 ELISA Kit, myd88-a ELISA Kit, myd88-b ELISA Kit, myeloid differentiation primary response 88 ELISA Kit, myeloid differentiation primary response 88 S homeolog ELISA Kit, myeloid differentiation primary response gene 88 ELISA Kit, CG2078 gene product from transcript CG2078-RB ELISA Kit, myeloid differentiation primary response protein MyD88-A ELISA Kit, myeloid differentiation primary response protein MyD88 ELISA Kit, myeloid differentiation factor 88 ELISA Kit, myeloid differentiation primary response 88 L homeolog ELISA Kit, MYD88 ELISA Kit, myd88.S ELISA Kit, Myd88 ELISA Kit, myd88 ELISA Kit, LOC413194 ELISA Kit, LOC100118553 ELISA Kit, LOC575153 ELISA Kit, myd88.L ELISA Kit

Hintergrund:

Gene Name: Myeloid Differentiation Factor 88

Gene Aliases: Myeloid Differentiation Primary Response Gene 88

Pathways: NF-kappaB Signalweg, TLR Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades