MIOX ELISA Kit

Produktnummer ABIN5657066

Produktdetails MIOX ELISA Kit

Target: MIOX (Myo-Inositol Oxygenase (MIOX))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Myo Inositol Oxygenase (MIOX). No significant cross-reactivity or interference between Myo Inositol Oxygenase (MIOX) and analogues was observed.

Sensitivität: 0.069 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myo Inositol Oxygenase (MIOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myo Inositol Oxygenase (MIOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myo Inositol Oxygenase (MIOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myo Inositol Oxygenase (MIOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myo Inositol Oxygenase (MIOX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myo Inositol Oxygenase (MIOX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MIOX

Target: MIOX (Myo-Inositol Oxygenase (MIOX))

Andere Bezeichnung: Myo Inositol Oxygenase (MIOX Produkte)

Synonyme: ALDRL6 ELISA Kit, 0610009I10Rik ELISA Kit, AI314022 ELISA Kit, Aldrl6 ELISA Kit, C85427 ELISA Kit, RSOR ELISA Kit, MGC82300 ELISA Kit, MGC86377 ELISA Kit, aldrl6 ELISA Kit, zgc:114168 ELISA Kit, DDBDRAFT_0188751 ELISA Kit, DDBDRAFT_0235367 ELISA Kit, DDB_0188751 ELISA Kit, DDB_0235367 ELISA Kit, MI oxygenase ELISA Kit, myo-inositol oxygenase ELISA Kit, myo-inositol oxygenase L homeolog ELISA Kit, inositol oxygenase 1 ELISA Kit, inositol oxygenase ELISA Kit, Myo-inositol oxygenase ELISA Kit, MIOX ELISA Kit, Miox ELISA Kit, miox.L ELISA Kit, miox ELISA Kit, MGG_09462 ELISA Kit, CNF03950 ELISA Kit, CC1G_10713 ELISA Kit, SCHCODRAFT_258535 ELISA Kit, CGB_F1290W ELISA Kit, mioX ELISA Kit, LOC100101177 ELISA Kit, LOC107131132 ELISA Kit

Hintergrund:

Gene Name: Myo Inositol Oxygenase

Gene Aliases: ALDRL6, KSP32, RSOR, Kidney-Specific Protein 32, Aldehyde Reductase(Aldose Reductase)Like 6, Renal-specific oxidoreductase, Myo-inositol oxygenase

Pathways: Inositol Metabolic Process