MYH11 ELISA Kit

Produktnummer ABIN5657088

Produktdetails MYH11 ELISA Kit

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 11, Smooth Muscle (MYH11). No significant cross-reactivity or interference between Myosin Heavy Chain 11, Smooth Muscle (MYH11) and analogues was observed.

Sensitivität: 0.27 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 11, Smooth Muscle (MYH11). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 11, Smooth Muscle (MYH11). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 11, Smooth Muscle (MYH11), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 11, Smooth Muscle (MYH11) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 11, Smooth Muscle (MYH11) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 11, Smooth Muscle (MYH11) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MYH11

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Andere Bezeichnung: Myosin Heavy Chain 11, Smooth Muscle (MYH11 Produkte)

Synonyme: AV071570 ELISA Kit, SM1 ELISA Kit, SM2 ELISA Kit, smMHC ELISA Kit, AAT4 ELISA Kit, FAA4 ELISA Kit, SMHC ELISA Kit, SMMHC ELISA Kit, myosin ELISA Kit, myh11 ELISA Kit, myosin, heavy polypeptide 11, smooth muscle ELISA Kit, myosin heavy chain 11 ELISA Kit, myosin, heavy chain 11, smooth muscle ELISA Kit, myosin, heavy chain 11a, smooth muscle ELISA Kit, Myh11 ELISA Kit, MYH11 ELISA Kit, myh11a ELISA Kit

Hintergrund:

Gene Name: Myosin Heavy Chain 11, Smooth Muscle

Gene Aliases: AAT4, FAA4, SMHC, SMMHC, Myosin heavy chain, smooth muscle isoform