NSF ELISA Kit

Produktnummer ABIN5657148

Produktdetails NSF ELISA Kit

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of N-Ethylmaleimide Sensitive Factor (NSF). No significant cross-reactivity or interference between N-Ethylmaleimide Sensitive Factor (NSF) and analogues was observed.

Sensitivität: 0.117 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Ethylmaleimide Sensitive Factor (NSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Ethylmaleimide Sensitive Factor (NSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Ethylmaleimide Sensitive Factor (NSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-Ethylmaleimide Sensitive Factor (NSF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-Ethylmaleimide Sensitive Factor (NSF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Andere Bezeichnung: N-Ethylmaleimide Sensitive Factor (NSF Produkte)

Synonyme: SKD2 ELISA Kit, AI316878 ELISA Kit, AU020090 ELISA Kit, AU067812 ELISA Kit, NSF ELISA Kit, 18.m06598 ELISA Kit, nsf ELISA Kit, si:bz18k17.1 ELISA Kit, wu:fj33g11 ELISA Kit, N-ethylmaleimide sensitive factor ELISA Kit, T1J1.4 ELISA Kit, T1J1_4 ELISA Kit, NEM-sensitive fusion protein ELISA Kit, N-ethylmaleimide sensitive factor, vesicle fusing ATPase ELISA Kit, N-ethylmaleimide sensitive fusion protein ELISA Kit, N-ethylmaleimide-sensitive factor ELISA Kit, N-ethylmaleimide sensitive factor ELISA Kit, N-ethylmaleimide-sensitive fusion protein, putative ELISA Kit, N-ethylmaleimide sensitive factor L homeolog ELISA Kit, vesicle-fusing ATPase ELISA Kit, N-ethylmaleimide-sensitive factor a ELISA Kit, AAA-type ATPase family protein ELISA Kit, NSF ELISA Kit, Nsf ELISA Kit, nsf ELISA Kit, BBOV_II007200 ELISA Kit, TGME49_318510 ELISA Kit, nsf.L ELISA Kit, LOC100180685 ELISA Kit, LOC9310886 ELISA Kit, nsfa ELISA Kit

Hintergrund:

Gene Name: N-Ethylmaleimide Sensitive Factor

Gene Aliases: SKD2, N-Ethylmaleimide Sensitive Fusion Protein, Vesicle-fusing ATPase, Vesicular-fusion protein NSF

Gen-ID: 4905

UniProt: P46459