NEIL1 ELISA Kit

Produktnummer ABIN5657200

Produktdetails NEIL1 ELISA Kit

Target: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Nei Endonuclease VIII Like Protein 1 (NEIL1). No significant cross-reactivity or interference between Nei Endonuclease VIII Like Protein 1 (NEIL1) and analogues was observed.

Sensitivität: 0.062 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nei Endonuclease VIII Like Protein 1 (NEIL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nei Endonuclease VIII Like Protein 1 (NEIL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nei Endonuclease VIII Like Protein 1 (NEIL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nei Endonuclease VIII Like Protein 1 (NEIL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nei Endonuclease VIII Like Protein 1 (NEIL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nei Endonuclease VIII Like Protein 1 (NEIL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu NEIL1

Target: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Andere Bezeichnung: Nei Endonuclease VIII Like Protein 1 (NEIL1 Produkte)

Synonyme: FPG1 ELISA Kit, NEI1 ELISA Kit, hFPG1 ELISA Kit, 2810450N13Rik ELISA Kit, Nei1 ELISA Kit, nei-like DNA glycosylase 1 ELISA Kit, nei like DNA glycosylase 1 ELISA Kit, nei endonuclease VIII-like 1 (E. coli) ELISA Kit, Neil1 ELISA Kit, NEIL1 ELISA Kit

Hintergrund:

Gene Name: Nei Endonuclease VIII Like Protein 1

Gene Aliases: NEI1, hFPG1, FPG1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Nei-like protein 1, DNA glycosylase/AP lyase Neil1

Gen-ID: 79661

UniProt: Q96FI4

Pathways: DNA Reparatur