SYNE2 ELISA Kit

Produktnummer ABIN5657231

Produktdetails SYNE2 ELISA Kit

Target: SYNE2 (Spectrin Repeat Containing, Nuclear Envelope 2 (SYNE2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Nesprin 2 (Nesp2). No significant cross-reactivity or interference between Nesprin 2 (Nesp2) and analogues was observed.

Sensitivität: 0.055 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nesprin 2 (Nesp2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nesprin 2 (Nesp2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nesprin 2 (Nesp2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nesprin 2 (Nesp2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nesprin 2 (Nesp2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nesprin 2 (Nesp2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SYNE2

Target: SYNE2 (Spectrin Repeat Containing, Nuclear Envelope 2 (SYNE2))

Andere Bezeichnung: Nesprin 2 (SYNE2 Produkte)

Synonyme: EDMD5 ELISA Kit, NUA ELISA Kit, NUANCE ELISA Kit, Nesp2 ELISA Kit, Nesprin-2 ELISA Kit, SYNE-2 ELISA Kit, TROPH ELISA Kit, SYNE2 ELISA Kit, nua ELISA Kit, edmd5 ELISA Kit, nuance ELISA Kit, syne-2 ELISA Kit, nesprin-2 ELISA Kit, 6820443O06Rik ELISA Kit, AW546258 ELISA Kit, D12Ertd777e ELISA Kit, Nesp2g ELISA Kit, mKIAA1011 ELISA Kit, RGD1305248 ELISA Kit, spectrin repeat containing nuclear envelope protein 2 ELISA Kit, spectrin repeat containing, nuclear envelope 2 ELISA Kit, SYNE2 ELISA Kit, syne2 ELISA Kit, Syne2 ELISA Kit

Hintergrund:

Gene Name: Nesprin 2

Gene Aliases: SYNE2, NUA, NUANCE, SYNE-2, Synaptic nuclear envelope protein 2, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein

Gen-ID: 23224

UniProt: Q8WXH0

Pathways: Maintenance of Protein Location