NEUROD6 ELISA Kit

Produktnummer ABIN5657278

Produktdetails NEUROD6 ELISA Kit

Target: NEUROD6 (Neurogenic Differentiation 6 (NEUROD6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Neurogenic Differentiation 6 (NEUROD6). No significant cross-reactivity or interference between Neurogenic Differentiation 6 (NEUROD6) and analogues was observed.

Sensitivität: 0.055 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurogenic Differentiation 6 (NEUROD6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurogenic Differentiation 6 (NEUROD6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurogenic Differentiation 6 (NEUROD6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurogenic Differentiation 6 (NEUROD6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurogenic Differentiation 6 (NEUROD6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurogenic Differentiation 6 (NEUROD6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu NEUROD6

Target: NEUROD6 (Neurogenic Differentiation 6 (NEUROD6))

Andere Bezeichnung: Neurogenic Differentiation 6 (NEUROD6 Produkte)

Synonyme: Atoh2 ELISA Kit, MATH2 ELISA Kit, Math-2 ELISA Kit, NEX1M ELISA Kit, Nex1 ELISA Kit, bHLHa2 ELISA Kit, nex1 ELISA Kit, Xath2 ELISA Kit, atoh2 ELISA Kit, math2 ELISA Kit, nex1m ELISA Kit, bhlha2 ELISA Kit, math-2 ELISA Kit, neurod6-A ELISA Kit, RGD1562793 ELISA Kit, MGC145341 ELISA Kit, Math2 ELISA Kit, Nex ELISA Kit, Nex1m ELISA Kit, atoh2b ELISA Kit, ndr1b ELISA Kit, zNdr1b ELISA Kit, zgc:92543 ELISA Kit, NeuroD6 ELISA Kit, NeuroD6-A ELISA Kit, Zath2a ELISA Kit, atoh2a ELISA Kit, ndr1a ELISA Kit, neuroD6-A ELISA Kit, neuronal differentiation 6 ELISA Kit, neuronal differentiation 6 L homeolog ELISA Kit, neurogenic differentiation 6 ELISA Kit, neuronal differentiation 6b ELISA Kit, neuronal differentiation 6a ELISA Kit, NEUROD6 ELISA Kit, neurod6.L ELISA Kit, Neurod6 ELISA Kit, neurod6 ELISA Kit, neurod6b ELISA Kit, neurod6a ELISA Kit

Hintergrund:

Gene Name: Neurogenic Differentiation 6

Gene Aliases: Atoh2, NEX1M, Math-2, bHLHa2, Neurogenic differentiation factor 6, Mammalian Atonal Homolog 2, Atonal Homolog 2, Class A basic helix-loop-helix protein 2