Neuromedin U ELISA Kit

Produktnummer ABIN5657294

Produktdetails Neuromedin U ELISA Kit

Target: Neuromedin U (NMU)

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Detektionsbereich: 123.5 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 123.5 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Neuromedin U (NMU). No significant cross-reactivity or interference between Neuromedin U (NMU) and analogues was observed.

Sensitivität: 48.2 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuromedin U (NMU) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuromedin U (NMU) and unlabeled Neuromedin U (NMU) (Standards or samples) with the pre-coated antibody specific to Neuromedin U (NMU). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuromedin U (NMU) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neuromedin U (NMU) in the sample.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuromedin U (NMU) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuromedin U (NMU) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu Neuromedin U

Target: Neuromedin U (NMU)

Andere Bezeichnung: Neuromedin U (NMU Produkte)

Synonyme: si:rp71-4m17.1 ELISA Kit, NmU-25 ELISA Kit, NMU ELISA Kit, neuromedin U ELISA Kit, NMU ELISA Kit, Nmu ELISA Kit, nmu ELISA Kit

Hintergrund:

Gene Name: Neuromedin U

Gene Aliases: NM-U

Gen-ID: 63887

UniProt: P12760

Pathways: Feeding Behaviour, Photoperiodism