NOX1 ELISA Kit
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- Target Alle NOX1 ELISA Kits anzeigen
- NOX1 (NADPH Oxidase 1 (NOX1))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.156 ng/mL - 10 ng/mL
- Untere Nachweisgrenze
- 0.156 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). No significant cross-reactivity or interference between Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) and analogues was observed.
- Sensitivität
- 0.061 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle NOX1 ELISA Kits anzeigen
- NOX1 (NADPH Oxidase 1 (NOX1))
- Andere Bezeichnung
- Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 (NOX1 Produkte)
- Synonyme
- GP91-2 ELISA Kit, MOX1 ELISA Kit, NOH-1 ELISA Kit, NOH1 ELISA Kit, NOX1a ELISA Kit, NOX1alpha ELISA Kit, Nox-1 ELISA Kit, Nox1 ELISA Kit, NADPH oxidase 1 ELISA Kit, NOX1 ELISA Kit, Nox1 ELISA Kit, nox1 ELISA Kit
- Hintergrund
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Gene Name: Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1
Gene Aliases: MOX1, GP91-2, NOH1, NADPH Oxidase 1, Mitogenic Oxidase(Pyridine Nucleotide-Dependent Superoxide-Generating, NADH/NADPH mitogenic oxidase subunit P65-MOX
- Gen-ID
- 27035
- UniProt
- Q9Y5S8
- Pathways
- Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport
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