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Nitrotyrosine ELISA Kit

Reaktivität: Diverse Spezies Colorimetric Competition ELISA 3.7 ng/mL - 300 ng/mL Plasma, Serum
Produktnummer ABIN5657415
  • Target Alle Nitrotyrosine ELISA Kits anzeigen
    Nitrotyrosine
    Reaktivität
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    Diverse Spezies
    Nachweismethode
    Colorimetric
    Methodentyp
    Competition ELISA
    Detektionsbereich
    3.7 ng/mL - 300 ng/mL
    Untere Nachweisgrenze
    3.7 ng/mL
    Applikation
    ELISA
    Proben
    Plasma, Serum
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Nitrotyrosine (NT). No significant cross-reactivity or interference between Nitrotyrosine (NT) and analogues was observed.
    Sensitivität
    1.35 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    2 h
    Plattentyp
    Pre-coated
    Protokoll
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Nitrotyrosine (NT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Nitrotyrosine (NT) and unlabeled Nitrotyrosine (NT) (Standards or samples) with the pre-coated antibody specific to Nitrotyrosine (NT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Nitrotyrosine (NT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Nitrotyrosine (NT) in the sample.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitrotyrosine (NT) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitrotyrosine (NT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle Nitrotyrosine ELISA Kits anzeigen
    Nitrotyrosine
    Abstract
    Nitrotyrosine Produkte
    Substanzklasse
    Chemical
    Hintergrund

    Gene Name: Nitrotyrosine

    Gene Aliases: 3-Nitro-L-Tyrosine, 3-Nitrotyrosine

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