NLRC4 ELISA Kit

Produktnummer ABIN5657418

Produktdetails NLRC4 ELISA Kit

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of NLR Family, CARD Domain Containing Protein 4 (NLRC4). No significant cross-reactivity or interference between NLR Family, CARD Domain Containing Protein 4 (NLRC4) and analogues was observed.

Sensitivität: 0.056 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLR Family, CARD Domain Containing Protein 4 (NLRC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NLR Family, CARD Domain Containing Protein 4 (NLRC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NLR Family, CARD Domain Containing Protein 4 (NLRC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NLR Family, CARD Domain Containing Protein 4 (NLRC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NLR Family, CARD Domain Containing Protein 4 (NLRC4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NLR Family, CARD Domain Containing Protein 4 (NLRC4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu NLRC4

Target: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Andere Bezeichnung: NLR Family, CARD Domain Containing Protein 4 (NLRC4 Produkte)

Synonyme: CARD12 ELISA Kit, CLAN ELISA Kit, CLAN1 ELISA Kit, CLANA ELISA Kit, CLANB ELISA Kit, CLANC ELISA Kit, CLAND ELISA Kit, CLR2.1 ELISA Kit, IPAF ELISA Kit, 9530011P19Rik ELISA Kit, Card12 ELISA Kit, NLR family CARD domain containing 4 ELISA Kit, NLR family, CARD domain containing 4 ELISA Kit, NLRC4 ELISA Kit, Nlrc4 ELISA Kit

Hintergrund:

Gene Name: NLR Family, CARD Domain Containing Protein 4

Gene Aliases: CARD12, CLAN1, CLANA, CLANB, CLANC, CLAND, CLR2.1, IPAF, Ice protease-activating factor, Caspase Recruitment Domain Family Member 12, NOD-Like Receptor C4

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome