NLRP3 CLIA Kit

Produktnummer ABIN5657419

Produktdetails NLRP3 CLIA Kit

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reaktivität: Human

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 781.25 pg/mL - 50000 pg/mL

Untere Nachweisgrenze: 781.25 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of NLR Family, Pyrin Domain Containing Protein 3 (NLRP3). No significant cross-reactivity or interference between NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) and analogues was observed.

Sensitivität: 27.5 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NLR Family, Pyrin Domain Containing Protein 3 (NLRP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Andere Bezeichnung: NLR Family, Pyrin Domain Containing Protein 3 (NLRP3 Produkte)

Synonyme: CIAS1 CLIA Kit, Nalp3 CLIA Kit, avp CLIA Kit, cias1 CLIA Kit, nalp3 CLIA Kit, pypaf1 CLIA Kit, agtavprl CLIA Kit, AGTAVPRL CLIA Kit, AII CLIA Kit, AVP CLIA Kit, C1orf7 CLIA Kit, CLR1.1 CLIA Kit, FCAS CLIA Kit, FCU CLIA Kit, MWS CLIA Kit, NALP3 CLIA Kit, PYPAF1 CLIA Kit, AII/AVP CLIA Kit, Cias1 CLIA Kit, Mmig1 CLIA Kit, Pypaf1 CLIA Kit, NLR family pyrin domain containing-like CLIA Kit, NLR family pyrin domain containing 3 CLIA Kit, NLR family, pyrin domain containing 3 L homeolog CLIA Kit, NLR family, pyrin domain containing 3 CLIA Kit, NLRPL CLIA Kit, NLRP3 CLIA Kit, nlrp3.L CLIA Kit, Nlrp3 CLIA Kit

Hintergrund:

Gene Name: NLR Family, Pyrin Domain Containing Protein 3

Gene Aliases: AVP, AGTAVPRL, AII, AII/AVP, C1orf7, CIAS1, CLR1.1, FCAS, FCU, MWS, NALP3, PYPAF1, Cryopyrin, Cold Induced Autoinflammatory Syndrome 1, Caterpiller-Like Receptor 1.1

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome