NONO ELISA Kit

Produktnummer ABIN5657428

Produktdetails NONO ELISA Kit

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Non POU Domain Containing Octamer Binding Protein (NONO). No significant cross-reactivity or interference between Non POU Domain Containing Octamer Binding Protein (NONO) and analogues was observed.

Sensitivität: 0.056 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Non POU Domain Containing Octamer Binding Protein (NONO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Non POU Domain Containing Octamer Binding Protein (NONO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Non POU Domain Containing Octamer Binding Protein (NONO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Non POU Domain Containing Octamer Binding Protein (NONO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Non POU Domain Containing Octamer Binding Protein (NONO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Non POU Domain Containing Octamer Binding Protein (NONO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu NONO

Target: NONO (Non-POU Domain Containing, Octamer-Binding (NONO))

Andere Bezeichnung: Non POU Domain Containing Octamer Binding Protein (NONO Produkte)

Synonyme: NMT55 ELISA Kit, NRB54 ELISA Kit, P54 ELISA Kit, P54NRB ELISA Kit, nmt55 ELISA Kit, nrb54 ELISA Kit, p54nrb ELISA Kit, xp54nrb ELISA Kit, AA407051 ELISA Kit, AV149256 ELISA Kit, nonA ELISA Kit, non-POU domain containing octamer binding ELISA Kit, non-POU domain containing, octamer-binding ELISA Kit, non-POU domain containing, octamer binding L homeolog ELISA Kit, non-POU-domain-containing, octamer binding protein ELISA Kit, NONO ELISA Kit, Nono ELISA Kit, nono.L ELISA Kit

Hintergrund:

Gene Name: Non POU Domain Containing Octamer Binding Protein

Gene Aliases: P54, NMT55, NRB54, P54NRB, Nuclear RNA-Binding Protein 54-kD, 54 kDa nuclear RNA- and DNA-binding protein, DNA-binding p52/p100 complex, 52 kDa subunit